Real-time dynamics of label-free single mast cell granules revealed by differential interference contrast microscopy
作者： Hung-Wing Li , Michael McCloskey , Yan He , Edward S. Yeung
DOI: 10.1007/S00216-006-0403-8
关键词:
摘要: We demonstrate the capability of differential interference contrast (DIC) microscopy as a simple and useful tool for studying cellular events without fluorescence labeling. By coupling an advanced DIC microscope to computer-controlled motorized vertical stage high-speed, high-resolution CCD camera, real-time three-dimensional monitoring is possible in high-throughput manner. The performance among three modes microscopy, bright-field, dark-field DIC, terms horizontal resolving power sectioning was investigated. As model, exocytosis rat peritoneal mast cells recorded on subsecond time scale. Three-dimensional tracking granules during degranulation achieved granule-granule fusion before plasma membrane recorded.
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