Ultrasonographic and histological characterization of the placenta of somatic nuclear transfer-derived pregnancies in dairy cattle

摘要: The high incidence of pregnancy loss and prenatal morbidity mortality in cloned animals may be due to placental insufficiency, thereby compromising fetal survival. Our objective was characterize morphological changes membranes bovine pregnancies. Two groups cows with fetuses, produced by two cloning techniques, a commercial group (n = 16) hand-made 4), control fetuses derived from traditional embryo transfer 6) or AI 6), were compared at various stages gestation (Days 80, 120, 150, 180, 210, 240; Day 0 estrus). Thickness shape the amniotic membrane, placentome length, umbilical cord diameter, fluid echodensities assessed ultrasonography, placenta evaluated histologically. Only eight (40%) pregnancies reached term seven calves (35%) alive birth. Both length diameter larger (P < 0.05) clones than normal all gestation. Amniotic membrane abnormalities (Day 120) including focal edema presence series nodules detected 38% always accompanied hyper-echodense spikes irregularities (detected ultrasonographically) around cord. Histopathology revealed degenerate inflammatory cells, edematous chorioallantoic membranes, decreased epithelial thickness. We inferred that these anomalies placentomes compromised development, we concluded ultrasonographic monitoring enabled characterization placentae useful assess well-being.