Cellular Distribution of Retinoic Acid Receptor-α Protein in Serous Adenocarcinomas of Ovarian, Tubal, and Peritoneal Origin

摘要: Retinoids are effective growth modulators of human ovarian carcinoma cell lines. Their effects mediated by nuclear retinoic acid receptors (RARs) and retinoid X (RXRs), which transcriptional factors members the steroid/thyroid receptor superfamily. To our knowledge, until now, cellular distribution RAR proteins in tumor specimens is unknown. This study provides new data on differential localization RARα protein 16 serous adenocarcinomas originating from ovaries, fallopian tubes, peritoneum. Using an affinity-purified antiserum specific for a monoclonal antibody recognizing full-length estrogen molecule (clone 6F11), we performed immunohistochemistry frozen tissue sections examined relationship between expression comparing percentage immunostained cells either receptor. Our findings indicate strong linear percentages RARα- receptor-labeled as determined regression analysis ( P r = 0.825). A modest inverse was found RARα-positive histological grade, attesting to differentiation-dependent trend 0.139), site origin (ovaries versus tubes peritoneum) 0.170), primary metastatic lesion 0.561). Thus, capable expressing despite high grade advanced stage neoplastic disease. Compared with heterogeneous cancer cells, there widespread immunoreactivity tumor-infiltrating lymphocytes, vascular endothelial stromal fibroblasts, underscoring value accurate determination RAR/(RXR) content specimens.