Effect of TGF-β1 Stimulation on the Secretome of Human Adipose-Derived Mesenchymal Stromal Cells
作者： Tania M. Rodríguez , Alejandro Saldías , Marcelo Irigo , Jorge Velasco Zamora , Marcelo J. Perone
关键词:
摘要: Adipose tissue is an attractive source of mesenchymal stromal cells (MSCs) owing to the relative ease obtaining large volumes with more MSC abundance compared other sources. Increasing evidence supports fact that trophic factors secreted by MSCs play a pivotal therapeutic role. Several strategies in regenerative medicine use MSCs, mainly exploiting their immunosuppressive effect and homing capacity sites damage. Transforming growth factor-β1 (TGF-β1) pleiotropic cytokine that, depending on cell niche, can display either anti-inflammatory or proinflammatory effects. TGF-β1 expression increases various tissues damage, especially when accompanied inflammation. Thus, we analyzed secretion adipose-derived (ASCs) panel 80 cytokines/chemokines using antibody array. To avoid possible fetal bovine serum (FBS) ASCs secretion, performed our analysis culturing FBS-free conditions, only supplemented 0.1% albumin. We report profile ASCs. also found exposure modulates 8 chemokines 18 cytokines, including -β2, important cytokines involved immunosuppression, allergic responses, bone resorption.
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