Flavonoids from Gynostemma pentaphyllum Exhibit Differential Induction of Cell Cycle Arrest in H460 and A549 Cancer Cells

摘要: Flavonoids, containing mainly kaempferol rhamnohexoside derivatives, were extracted from Gynostemma pentaphyllum (G. pentaphyllum) and their potential growth inhibition effects against H460 non-small cell lung cancer cells was explored compared to that on A549 cells. The flavonoids found exhibit antiproliferation (IC50 = 50.2 μg/mL), although the IC50 of is 2.5-fold 19.8 μg/mL). Further investigation revealed are more susceptible than A549, whereas better inhibited by derivatives as with H460. In addition, G. induced cycle arrest at both S G2/M phases concurrent modulated expression cellular proteins cyclin A, B, p53 p21 in cells, but not On contrary, apoptosis concomitant alteration balance BCL-2 BAX well activation caspase-3 equally affected between flavonoid treatment. These observations strongly suggest discrepancy following treatment can be attributed lack differences may serve contrasting models for further mechanistic investigations.