The Adaptor Protein Complex 1 limits E-cadherin endocytosis during epithelial morphogenesis
作者： Miguel Ramírez Moreno , Katy Boswell , Natalia A. Bulgakova
DOI: 10.1101/2020.10.14.340372
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摘要: Abstract Intracellular trafficking regulates the distribution of transmembrane proteins including key determinants epithelial polarity and adhesion. The Adaptor Protein 1 (AP-1) complex is regulator vesicle sorting, which binds a large number specific cargos. We examined roles AP-1 in morphogenesis, using Drosophila wing as paradigm. found that knockdown leads to ectopic folds caused by defects integrins. This occurs concurrently with an increase apical cell area induction death due E-cadherin trafficking. discovered distinct pool localizes at Adherens Junctions, where it limits internalization from surface. Upon knockdown, accompanying hyperinternalization induces uncharacterised mechanism potential tumour-suppressive role. Simultaneously, cells expression compensatory maintain cell-cell
sci-hub.st 参考文章(64)
Alexey Larionov, Andreas Krause, William Miller, A standard curve based method for relative real time PCR data processing BMC Bioinformatics. ,vol. 6, pp. 62- 68 ,(2005) , 10.1186/1471-2105-6-62
Andrew P. Kowalczyk, Benjamin A. Nanes, Adherens Junction Turnover: Regulating Adhesion Through Cadherin Endocytosis, Degradation, and Recycling Subcellular Biochemistry. ,vol. 60, pp. 197- 222 ,(2012) , 10.1007/978-94-007-4186-7_9
Kenneth M Yamada, Shingo Miyamoto, Integrin transmembrane signaling and cytoskeletal control Current Opinion in Cell Biology. ,vol. 7, pp. 681- 689 ,(1995) , 10.1016/0955-0674(95)80110-3
Tony J.C. Harris, Mark Peifer, Adherens junction-dependent and -independent steps in the establishment of epithelial cell polarity in Drosophila Journal of Cell Biology. ,vol. 167, pp. 135- 147 ,(2004) , 10.1083/JCB.200406024
M. D. Martin-Bermudo, N. H. Brown, Uncoupling integrin adhesion and signaling: the βPS cytoplasmic domain is sufficient to regulate gene expression in the Drosophila embryo Genes & Development. ,vol. 13, pp. 729- 739 ,(1999) , 10.1101/GAD.13.6.729
Barbara Jezowska, Beatriz García Fernández, Ana Rita Amândio, Paulo Duarte, Cláudia Mendes, Catarina Brás-Pereira, Florence Janody, A dual function of Drosophila capping protein on DE-cadherin maintains epithelial integrity and prevents JNK-mediated apoptosis Developmental Biology. ,vol. 360, pp. 143- 159 ,(2011) , 10.1016/J.YDBIO.2011.09.016
Christian Bökel, Nicholas H. Brown, Integrins in development: moving on, responding to, and sticking to the extracellular matrix. Developmental Cell. ,vol. 3, pp. 311- 321 ,(2002) , 10.1016/S1534-5807(02)00265-4
Koji Hase, Fubito Nakatsu, Masumi Ohmae, Kazushi Sugihara, Noriko Shioda, Daisuke Takahashi, Yuuki Obata, Yukihiro Furusawa, Yumiko Fujimura, Taisuke Yamashita, Shinji Fukuda, Hiroshi Okamoto, Masahide Asano, Shigenobu Yonemura, Hiroshi Ohno, AP-1B−Mediated Protein Sorting Regulates Polarity and Proliferation of Intestinal Epithelial Cells in Mice Gastroenterology. ,vol. 145, pp. 625- 635 ,(2013) , 10.1053/J.GASTRO.2013.05.013
Valerie C. Coffman, Jian-Qiu Wu, Counting protein molecules using quantitative fluorescence microscopy Trends in Biochemical Sciences. ,vol. 37, pp. 499- 506 ,(2012) , 10.1016/J.TIBS.2012.08.002
E. M. M. MANDERS, F. J. VERBEEK, J. A. ATEN, Measurement of co-localization of objects in dual-colour confocal images Journal of Microscopy. ,vol. 169, pp. 375- 382 ,(1993) , 10.1111/J.1365-2818.1993.TB03313.X