Adherens junction-dependent and -independent steps in the establishment of epithelial cell polarity in Drosophila
作者： Tony J.C. Harris , Mark Peifer
关键词: Biology 、 Actin 、 Adherens junction 、 Cadherin 、 Cytoskeleton 、 Epithelial polarity 、 Gastrulation 、 Cell polarity 、 Drosophila Protein 、 Cell biology
摘要: Adherens junctions (AJs) are thought to be key landmarks for establishing epithelial cell polarity, but the origin of polarity in Drosophila remains unclear. Thus, we examined establishment during early development. We found apical accumulation both E-Cadherin (DE-Cad) and cue Bazooka (Baz) as cells first form. Mutant analyses revealed that Baz accumulations can established absence AJs, whereas assembly DE-Cad complexes requires Baz. acts upstream AJs establishment. During gastrulation results widespread dissociation depolarization. Some structures retained, however. These maintain Baz, accumulate Crumbs, organize polarized cytoskeletons, display abnormal morphology fail segregate basolateral Discs large from domain. although develops play specific roles maintaining architecture segregating cues.
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