Defective XRN3-mediated transcription termination in Arabidopsis affects the expression of protein-coding genes.
作者： Norbert Dojer , Wojciech Karlowski , Joanna Kufel , Michal Krzyszton , Monika Zakrzewska‐Placzek
DOI: 10.1111/TPJ.13826
关键词:
摘要: Arabidopsis thaliana contains two nuclear XRN2/3 5'-3' exonucleases that are homologs of yeast and human Rat1/Xrn2 proteins involved in the processing degradation several classes RNAs transcription termination RNA polymerase II. Using strand-specific short read sequencing we show knockdown XRN3 leads to an altered expression hundreds genes accumulation uncapped polyadenylated read-through transcripts generated by inefficiently terminated Pol Our data support notion XRN3-mediated changes a subset caused upstream these effects enhanced RNA-mRNA chimeras xrn3 plants. In turn, antisense downstream may trigger production siRNA. results highlight importance exoribonuclease II plants disturbance this process significantly alter gene expression.
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