HUMAN THIOPURINE METHYLTRANSFERASE: NO EVIDENCE OF ACTIVATION BY ITS SUBSTRATES
作者： Ieva Stupans , Amra Kirlich , Ross A McKinnon
DOI: 10.1016/S0024-3205(97)01116-8
关键词:
摘要: A HPLC assay was developed to baculovirus expressed human thiopurine methyltransferase activity. Using 6-mercaptopurine as substrate, the found have an apparent Km of 0.99 mM and a Vmax 19 nmoles/mg/min. These values are in agreement with those determined using standard radiometric for The effects 6-thioguanine on metabolism were determined. 6-Thioguanine be mixed inhibitor methylation.
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