Selection of thermotolerant yeast strains Saccharomyces cerevisiae for bioethanol production

摘要: Abstract The simultaneous saccharification and fermentation (SSF) process of lignocellulosic materials requires the utilization microorganisms capable working at high temperatures. selection Saccharomyces cerevisiae strains able to ferment sugars obtained from material temperatures above 35 °C with ethanol yield has become a necessity. In this work, 11 S. were screened for their ability grow glucose in temperature range 35–45 °C. All grew (in agar plates) 35 40 °C, only two 42 °C, no strain 45 °C. yeasts placed liquid medium, incubated 35, 40 42 °C. Glucose-to-ethanol conversion was between 50% 80% theoretical value. Strains that produced least 70% submitted acclimatization treatments. One pure yeast isolated (IR2-9a). For bleached kraft pulp as well organosolv-pretreated Pinus radiata chips, yields by SSF using IR2-9a higher than those control yeast.