Cytogenetic effects of alachlor and/or atrazine in vivo and in vitro

摘要: The purpose of this study was to assess the cytogenetic effects two commonly used herbicides, alachlor and atrazine, which are often found together in groundwater. Chromosome damage examined bone marrow cells mice drinking water containing 20 ppm and/or with an immunosuppressive dose cyclophosphamide as a positive control. also quantified human lymphocytes exposed culture 1.0, 0.1, or 0.01 microgram/ml atrazine. vitro demonstrated related not associated mitotic inhibition cell death, due alachlor-atrazine combination suggesting additive model. vivo suggested alachloratrazine after 30 days treatment, but, unexpectedly, less fewer multiple aberrations 90 days. Also, at days, all treated had elevated indices compared controls. fact that index immune suppression group suggests death accumulated chromosomal resulted increased proliferation, so higher fraction were newer damage. Since showed little systemic toxicity despite similar animals, well decrease replacement must be involved but cannot completely explain results.