Electrochemical monitoring of the interaction of UO22 + with immobilized DNA
作者： Sima Pourbeyram , Reza Karimi Shervedani
DOI: 10.1016/J.BIOELECHEM.2013.02.002
关键词:
摘要: Interaction of uranyl ion (UO2(2+)) with immobilized double strand calf thymus DNA (ds-ct-DNA) on zirconium attached gold-mercaptopropionic acid self-assembled monolayer (Au-MPA-Zr(IV) SAM) is monitored by electrochemical techniques. The results show that after 15 min proximity the UO2(2+), peak currents square wave voltammograms are decreased (about 80%), and equivalent circuit model impedance spectroscopy (EIS) data, requires two constant phase elements (CPE) instead only one. By using surface concentration (≈2.3×10(-13) mol/cm(2)) number anthraquinonedisulfonic (AQDS) adsorbed (1.34×10(-10) ration AQDS per base pairs obtained ≈1/30 before to UO2(2+). Based EIS technique, we find structure electrode has been changed (damaged) This modified potential becoming a screening tool for rapid assessment interaction genotoxicity existing new chemicals.
elsevier.com
sci-hub.se 参考文章(23)
Impedance spectroscopy : theory, experiment, and applications Wiley-interscience. ,(2005) , 10.1002/0471716243
Peter E. Nielsen, Niels Erik Møllegaard, Claus Jeppesen, DNA Conformational analysis in solution by uranyl mediated photocleavage Nucleic Acids Research. ,vol. 18, pp. 3847- 3851 ,(1990) , 10.1093/NAR/18.13.3847
Reza Karimi Shervedani, Seyed Ahmad Mozaffari, Impedimetric sensing of uranyl ion based on phosphate functionalized cysteamine self-assembled monolayers Analytica Chimica Acta. ,vol. 562, pp. 223- 228 ,(2006) , 10.1016/J.ACA.2006.01.046
Tal M. Nahir, Edmond F. Bowden, Measurement of the Rate of Adsorption of Electroactive Cytochrome c to Modified Gold Electrodes by Electrochemical Impedance Spectroscopy Langmuir. ,vol. 18, pp. 5283- 5286 ,(2002) , 10.1021/LA020144M
Reza Karimi Shervedani, Sima Pourbeyram, Zirconium immobilized on gold-mercaptopropionic acid self-assembled monolayer for trace determination of phosphate in blood serum by using CV, EIS, and OSWV Biosensors and Bioelectronics. ,vol. 24, pp. 2199- 2204 ,(2009) , 10.1016/J.BIOS.2008.11.027
Digby D. Macdonald, Reflections on the history of electrochemical impedance spectroscopy Electrochimica Acta. ,vol. 51, pp. 1376- 1388 ,(2006) , 10.1016/J.ELECTACTA.2005.02.107
Niels Erik Møllegaard, Søren Lindemose, Peter E. Nielsen, Uranyl photoprobing of nonbent A/T- and bent A-tracts. A difference of flexibility? Biochemistry. ,vol. 44, pp. 7855- 7863 ,(2005) , 10.1021/BI0502083
Ziping Zhang, Jinyan Zhou, Aman Tang, Zhaoyang Wu, Guoli Shen, Ruqin Yu, Scanning electrochemical microscopy assay of DNA based on hairpin probe and enzymatic amplification biosensor Biosensors and Bioelectronics. ,vol. 25, pp. 1953- 1957 ,(2010) , 10.1016/J.BIOS.2010.01.013
Reza Karimi Shervedani, Sima Pourbeyram, Electrochemical determination of calf thymus DNA on Zr(IV) immobilized on gold–mercaptopropionic-acid self-assembled monolayer Bioelectrochemistry. ,vol. 77, pp. 100- 105 ,(2010) , 10.1016/J.BIOELECHEM.2009.07.001
Michael Weinfeld, Krista June M. Soderlind, 32P-postlabeling detection of radiation-induced DNA damage: identification and estimation of thymine glycols and phosphoglycolate termini. Biochemistry. ,vol. 30, pp. 1091- 1097 ,(1991) , 10.1021/BI00218A031