Ovarian structure protein 1: A sensitive molecular biomarker of gonadal intersex in female Japanese medaka after androgen exposure
作者： Ahmed Abdel-Moneim , Cecon T. Mahapatra , Azadeh Hatef , Maria S. Sepúlveda
DOI: 10.1002/ETC.3032
关键词:
摘要: Intersex in gonochoristic fish can be induced after exposure to androgens and estrogens. The main objective of the present study was identify biomarkers that would predictive intersex Japanese medaka (Oryzias latipes) synthetic hormones. First a gene identified, ovarian structure protein 1 (osp1), with strong female-specific expression during gonadal differentiation. authors hypothesized osp1 decrease male levels females larvae (15–25 d postfertilization [dpf]) 17β-trenbolone (TRB; 5 ng/L) increase female males exposed 17α-ethinylestradiol (EE2; later life (60 dpf). Tissue distribution cellular localization OSP1 investigated using Western blot immunohistochemistry. results indicate this regime delays testicular maturation development females. Although decreased TRB correlated changes phenotype, up-regulation not observed EE2. In addition, only ovaries localized cytoplasm follicular layer immature mature oocytes. conclude is promising biomarker androgen medaka. Environ Toxicol Chem 2015;34:2087–2094. © 2015 SETAC
sci-hub.se 参考文章(34)
充男 茅野, VIII 食糧生産と環境 1. 開会にあたって Environmental Sciences. ,vol. 11, pp. 221- 227 ,(1998)
Jessica K. Leet, Heather E. Gall, Maria S. Sepúlveda, A review of studies on androgen and estrogen exposure in fish early life stages: effects on gene and hormonal control of sexual differentiation. Journal of Applied Toxicology. ,vol. 31, pp. 379- 398 ,(2011) , 10.1002/JAT.1682
Valerio Matozzo, François Gagné, Maria Gabriella Marin, Francesco Ricciardi, Christian Blaise, Vitellogenin as a biomarker of exposure to estrogenic compounds in aquatic invertebrates: a review. Environment International. ,vol. 34, pp. 531- 545 ,(2008) , 10.1016/J.ENVINT.2007.09.008
Jon J. Amberg, Reuben Goforth, Tom Stefanavage, Maria S. Sepúlveda, Sexually dimorphic gene expression in the gonad and liver of shovelnose sturgeon (Scaphirhynchus platorynchus) Fish Physiology and Biochemistry. ,vol. 36, pp. 923- 932 ,(2010) , 10.1007/S10695-009-9369-8
Tohru Kobayashi, Masaru Matsuda, Hiroko Kajiura-Kobayashi, Aya Suzuki, Noriko Saito, Masatoshi Nakamoto, Naoki Shibata, Yoshitaka Nagahama, Two DM domain genes, DMY and DMRT1, involved in testicular differentiation and development in the medaka, Oryzias latipes Developmental Dynamics. ,vol. 231, pp. 518- 526 ,(2004) , 10.1002/DVDY.20158
Lynn P. Weber, Gordon C. Balch, Chris D. Metcalfe, David M. Janz, Increased kidney, liver, and testicular cell death after chronic exposure to 17α‐ethinylestradiol in medaka (Oryzias latipes) Environmental Toxicology and Chemistry. ,vol. 23, pp. 792- 797 ,(2004) , 10.1897/02-570
Tapas Chakraborty, Yasushi Shibata, Lin-Yan Zhou, Yoshinao Katsu, Taisen Iguchi, Yoshitaka Nagahama, Differential expression of three estrogen receptor subtype mRNAs in gonads and liver from embryos to adults of the medaka, Oryzias latipes. Molecular and Cellular Endocrinology. ,vol. 333, pp. 47- 54 ,(2011) , 10.1016/J.MCE.2010.12.002
Kathleen M. Jensen, Elizabeth A. Makynen, Michael D. Kahl, Gerald T. Ankley, Effects of the Feedlot Contaminant 17α-Trenbolone on Reproductive Endocrinology of the Fathead Minnow Environmental Science & Technology. ,vol. 40, pp. 3112- 3117 ,(2006) , 10.1021/ES052174S
Robert H. Devlin, Yoshitaka Nagahama, Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences Aquaculture. ,vol. 208, pp. 191- 364 ,(2002) , 10.1016/S0044-8486(02)00057-1
Yann Guiguen, Alexis Fostier, Francesc Piferrer, Ching-Fong Chang, Ovarian aromatase and estrogens: A pivotal role for gonadal sex differentiation and sex change in fish General and Comparative Endocrinology. ,vol. 165, pp. 352- 366 ,(2010) , 10.1016/J.YGCEN.2009.03.002