Alterations in macromolecular composition and cell wall integrity by ciprofloxacin in Mycobacterium smegmatis.
作者： I Verma , A Rohilla , GK Khuller , None
DOI: 10.1046/J.1365-2672.1999.00597.X
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摘要: I. VERMA, A. ROHILLA AND G.K. KHULLER. 1999.The present study has been undertaken to explore the biochemical mechanism of antimycobacterial action a potent fluoroquinolone i.e. ciprofloxacin in Mycobacterium smegmatis. Cells grown presence subinhibitory concentration (IC50) had significantly lower content all major macromolecules i.e DNA, RNA, proteins and lipids with maximum inhibition DNA as compared control. Significant quantitative changes were also observed various chemical constituents cell wall cells. A decrease number binding sites for fluorescent probe L-anilinonapthalene-8-sulphonate (ANS) cells suggested structural on surface. morphology by scanning electron microscopy (SEM). Our results suggest that exerts its activity affecting well macromolecules, particularly vital component survival growth.
sci-hub.se 参考文章(27)
Christopher S. Frings, Ralph T. Dunn, A colorimetric method for determination of total serum lipids based on the sulfo-phospho-vanillin reaction. American Journal of Clinical Pathology. ,vol. 53, pp. 89- 91 ,(1970) , 10.1093/AJCP/53.1.89
A. Dalhoff, Interaction of Aminoglycosides and Ciprofloxacin with Bacterial Membranes Springer, Berlin, Heidelberg. pp. 16- 27 ,(1985) , 10.1007/978-3-642-70748-3_2
Patrick J. Brennan, Philip Draper, Ultrastructure of Mycobacterium tuberculosis American Society of Microbiology. pp. 271- 284 ,(1994) , 10.1128/9781555818357.CH19
J C Wang, DNA topoisomerases: why so many? Journal of Biological Chemistry. ,vol. 266, pp. 6659- 6662 ,(1991) , 10.1016/S0021-9258(20)89545-3
Kenji Takeya, Kazuhito Hisatsune, Yasuko Inoue, MYCOBACTERIAL CELL WALLS II. Journal of Bacteriology. ,vol. 85, pp. 24- 30 ,(1963) , 10.1128/JB.85.1.24-30.1963
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
K. Burton, A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid Biochemical Journal. ,vol. 62, pp. 315- 323 ,(1956) , 10.1042/BJ0620315
H. E. Takiff, M. Cimino, M. C. Musso, T. Weisbrod, R. Martinez, M. B. Delgado, L. Salazar, B. R. Bloom, W. R. Jacobs, Efflux pump of the proton antiporter family confers low-level fluoroquinolone resistance in Mycobacterium smegmatis. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 93, pp. 362- 366 ,(1996) , 10.1073/PNAS.93.1.362
N Mor, J Vanderkolk, L Heifets, Inhibitory and bactericidal activities of levofloxacin against Mycobacterium tuberculosis in vitro and in human macrophages. Antimicrobial Agents and Chemotherapy. ,vol. 38, pp. 1161- 1164 ,(1994) , 10.1128/AAC.38.5.1161
Jan Slavik, Jaroslav Horák, Ludmila Říhová, Arnošt Kotyk, Anilinonaphthalene sulfonate fluorescence and amino acid transport in yeast. The Journal of Membrane Biology. ,vol. 64, pp. 175- 179 ,(1982) , 10.1007/BF01870883