Adhesion of Arg-Gly-Asp (RGD) Peptide Vesicles onto an Integrin Surface: Visualization of the Segregation of RGD Ligands into the Adhesion Plaques by Fluorescence

摘要: Integrins are adhesion receptors that mediate cell and play an important function in many biological processes such as morphogenesis tissue remodeling. These membrane proteins specifically interact with a short tripeptide sequence, RGD (Arg-Gly-Asp), present numerous extracellular macromolecules. Model systems have been developed order to understand how is induced by this specific peptide ligand/integrin recognition system. We previously shown giant vesicles selectively adhere endothelial cells formation of pinning centers. Nevertheless, the nature lipids located contact zone unknown. One hypothesis lipidic ligands migrate where they confined after binding receptor. To study possible ligand domains within vesicle bilayer, we synthesized fluorescently labeled lipid can be easily incorporated vesicles. Adhesion onto a...