A Picea abies linkage map based on SNP markers identifies QTLs for four aspects of resistance to Heterobasidion parviporum infection.

摘要: A consensus linkage map of Picea abies, an economically important conifer, was constructed based on the segregation 686 SNP markers in a F1 progeny population consisting 247 individuals. The total length 1889.2 cM covered 96.5% estimated genome and comprised 12 large groups, corresponding to number haploid P. abies chromosomes. sizes groups (from 5.9 9.9% length) correlated well with previous estimates chromosome 5.8 10.8% size). Any locus has 97% probability be within 10 from mapped marker, which makes suited for QTL mapping. Infecting trees root rot pathogen Heterobasidion parviporum allowed mapping four different resistance traits: lesion at inoculation site, fungal spread sapwood, exclusion host after initial infection, ability prevent infection establishing all. These traits were associated two, four, three regions respectively none overlapped between traits. Each explained 4.6 10.1% respective phenotypic variation. Although contain many more genes than ones represented by markers, least confidence intervals originated known function conifer defence; leucoanthocyanidine reductase, previously been shown upregulate during H. intermediates lignification process; hydroxycinnamoyl CoA shikimate/quinate hydroxycinnamoyltransferase, 4-coumarate ligase, R2R3-MYB transcription factor.