Infection of human primary hepatocytes with dengue virus serotype 2
作者： Lukkana Suksanpaisan , Arturo Cabrera-Hernandez , Duncan R. Smith
DOI: 10.1002/JMV.20798
关键词:
摘要: While the impact of dengue viruses on liver function is prominent as shown by hepatomegaly, enzyme abnormality, occasional fulminant hepatic failure and histological changes including hepatocellular necrosis, significant debate exists to possible involvement predominant cell type in liver, hepatocytes, disease process. To address this issue purified human primary hepatocytes were exposed virus serotype 2 production de novo viral progeny was established standard plaque assay, RT-PCR immunocytochemistry. investigate response infection, expression a panel 9 cytokine genes (IFN-beta, TRAIL, MCP-1, IL-6, IL-1beta, IL-8, MIP-1alpha, MIP-1beta, RANTES) semi-quantitatively investigated up-regulation IFN-beta, RANTES observed infection. The induction IL-8 infection accompanied secretion verified ELISA assay. ability be infected with vitro support evidence implicating target cases provide first experimental large number clinical studies that implicate critical organ severe
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