Patterning of angiogenesis in the zebrafish embryo.

摘要: Little is known about how vascular patterns are generated in the embryo. The vasculature of zebrafish trunk has an extremely regular pattern. One intersegmental vessel (ISV) sprouts from aorta, runs between each pair somites, and connects to dorsal longitudinal anastomotic (DLAV). We now define cellular origins, migratory paths cell fates that generate these metameric vessels trunk. Additionally, by a genetic screen we one gene, out bounds (obd), constrains this angiogenic growth specific path. have performed lineage analysis, using laser activation caged dye mosaic construction determine origin cells constitute ISV. Individual angioblasts destined for ISVs arise lateral posterior mesoderm (LPM), migrate where they somites their final position Cells ISV leave aorta only ventral regions two adjacent dorsally assume three fates. Most T-shaped cell, based DLAV branching ventrally; second constitutes connecting cell; third inverted dorsally. remains during its course, but changes run mid-somite This suggests pattern ventrally guided different cues. also ENU mutagenesis 750 mutagenized genomes identified mutation, obd disrupts In mutant embryos, sprout precociously at abnormal sites anomalously vicinity somite. extent less perturbed. Precocious sprouting can be inhibited VEGF morphant, anomalous site remains. somite causes wild-type endothelial embryos. Thus, launching new initial trajectory directed inhibitory signals somites. Zebrafish tractable system defining origins vessels, dissecting elements govern growth.