Tumor necrosis factor-alpha-based gene therapy enhances radiation cytotoxicity in human prostate cancer.

摘要: The purpose of the present study was to determine therapeutic potential combining radiotherapy with tumor necrosis factor (TNF)-alpha-based gene therapy in human prostate cancer PC-3 xenograft. cells are highly resistant TNF-alpha-induced cytotoxicity vitro. A modest enhancement radiation killing observed addition TNF-alpha clonogenic survival assays. Combined treatment Ad.Egr-TNF, a replication-deficient adenovirus modified express following exposure infected ionizing (40 Gy administered at 5 per fraction) vivo, resulted increased control, as defined by reduction volume, when compared Ad.Egr-TNF alone or (P < .03). improvement control achieved without increasing acute normal tissue damage injury from alone. results these studies support further development and clinical application genetic for cancer.