Investigating the importance of flow when utilizing hyaluronan scaffolds for tissue engineering.

摘要: Esterified hyaluronan scaffolds offer significant advantages for tissue engineering. They are recognized by cellular receptors, interact with many other extracellular matrix proteins and their metabolism is mediated intrinsic pathways. In this study differences in the viability structural integrity of vascular models cultured on under laminar flow conditions highlighted potential biodegradation kinetics, processes end-products, depending culture environment. Critical factors likely to include seeding densities duration magnitude applied biomechanical stress. Proteomic evaluation timing amount remodelling protein expression, resulting changes arising from response metabolic cell assay, together examination morphology, were conducted esterified felt PTFE mesh scaffolds. The derived using complete sheets harvested expanded umbilical cord vein cells. This method utilizes high-density populations outset, while cells already supported own abundant matrix. Type I type IV collagen expression parallel MMP-1 MMP-2 monitored over a 10 day period regimes immobilization technologies. Uniaxial tensile testing scanning electron microscopy used compare effects hydrodynamic stimulation upon integrity, assays evaluate shear function. proteomic results showed that felt-supported tissues expressed higher levels all than those mesh. Overall, 21% greater collagen, 24% 34% more observed during was coupled loss these after introduction flow, as compared increases mechanical properties mesh-supported tissues. However, conditions, hyaluronan-supported some recovery originally lost static contrast mesh-based models, where initial gains followed decline Proteomic, data emphasized need extended vitro evaluations enable better understanding multi-stage reparative biodegradable also possibility component, dynamic may be conducive optimal development environment because they facilitate efficient removal high concentrations degradation end-products accumulating pericellular space. Copyright © 2009 John Wiley & Sons, Ltd.