Fullerenol C60(OH)36 protects human erythrocyte membrane against high-energy electrons.
作者： Jacek Grebowski , Paulina Kazmierska , Grzegorz Litwinienko , Anna Lankoff , Marian Wolszczak
DOI: 10.1016/J.BBAMEM.2018.05.005
关键词:
摘要: Abstract Fullerenols (polyhydroxylated fullerene C60) are nanomaterial with potentially broad applicability in biomedical sciences high antioxidant ability, thus, we investigated the radioprotecting potential of fullerenol C60(OH)36 on human erythrocytes irradiated by high-energy electrons 6 MeV. The results demonstrate that at concentration 150 μg/mL protects against radiation-induced hemolysis (comparing to non-protected cells, observed 30% and 39% protection for 0.65 1.3 kGy irradiation doses, respectively). protecting effect was confirmed 32% decreased release potassium cations comparing cells without C60(OH)36. Measurements amount lactate dehydrogenase (LDH) released from showed size pores formed not sufficient LDH across erythrocyte membranes. We also report a significant decrease affinity acetylcholinesterase (AChE) substrate presence fullerenol, indicating relatively strong adsorption components plasma membrane. Changes membrane fluidity detected fluorescence spectroscopy conformational changes proteins spin labeling suggest dose-dependent formation disulfide groups as an oxidation this process inhibited suppose scavenging ROS well steric -SH responsible effects.
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