Lgr5-Expressing Cells Are Sufficient and Necessary for Postnatal Mammary Gland Organogenesis
作者： Vicki Plaks , Audrey Brenot , Devon A. Lawson , Jelena R. Linnemann , Eline C. Van Kappel
DOI: 10.1016/J.CELREP.2012.12.017
关键词:
摘要: Mammary epithelial stem cells are vital to tissue expansion and remodeling during various phases of postnatal mammary development. Basal enriched in Wnt-responsive can reconstitute cleared fat pads upon transplantation into mice. Lgr5 is a Wnt-regulated target gene was identified as major cell marker the small intestine, colon, stomach, hair follicle, well kidney nephrons. Here, we demonstrate outstanding regenerative potential rare population Lgr5-expressing (Lgr5(+)) (MECs). We found that Lgr5(+) reside within basal population, superior other regenerating functional glands (MGs), exceptionally efficient reconstituting MGs from single cells, exhibit capacity serial transplantations. Loss-of-function depletion experiments transplanted MECs or pubertal revealed these not only sufficient but also necessary for organogenesis.
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