Characterization of a neurite outgrowth inhibitor expressed after CNS injury.

摘要: Reactive gliosis, a general response to injury in the central nervous system grey and white matter, represents serious obstacle axonal regeneration mammals. In culture, myelin-free plasma membranes from normal rat brain tissue promoted neurite outgrowth, whereas purified injured were inhibitory. The inhibitory activity could be solubilized by detergent, was sensible glycosaminoglycan lyase digestion eluted with an apparent molecular weight of 160 – 220 kDa gel filtration chromatography. When presented as surface-bound molecule, inhibitor prevented initiation; when added soluble form growing neurites, it induced their retraction. These results provide cellular evidence supporting classical view that, mammalian system, damage-evoked gliosis correlates expression molecules capable preventing outgrowth.