In Vitro Luteinizing Hormone-Releasing Hormone Release from Superfused Rat Hypothalami: Site of Action of Progesterone and Effect of Estrogen Priming*
作者： K. KIM , V. D. RAMIREZ
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摘要: The study examined the effect of estrogen priming on progesterone (P4)-induced LHRH release, tissue site action P4, and 5α-dihydroxyprogesterone (5α- DHP) release from hypothalamic fragments superfused in vitro. Immature female rats were ovariectomized (OVX) and, at 28 days age, Silastic capsules containing estradiol (E2) implanted. Two later, animals killed removed transferred to superfusion chambers. units received P4 or 5α-DHP delivered an intermittent mode (10-min on, 20-min off). was determined perfusates by RIA. After input characteristics different infusion modes (single pulses, intermittent, continuous) infused into chambers assessed, off) selected for further examinations. In mediobasal hypothalamic- anterior hypothalamic-preoptic area (MBH-AHAPOA) preparations, we observed: 1) infusio...
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