Enhanced antifungal activity of voriconazole-loaded nanostructured lipid carriers against Candida albicans with a dimorphic switching model.

摘要: Candida commonly adheres to implanted medical devices and forms biofilms. Due the minimal activity of current antifungals against biofilms, new drugs or drug-delivery systems treat these persistent infections are urgently needed. In present investigation, voriconazole-loaded nanostructured lipid carriers (Vrc-NLCs) were formulated for enhanced efficiency C. albicans increase antifungal Vrc improve treatment infectious diseases. Vrc-NLCs prepared by a hot-melt, high-pressure homogenization method, size distribution, ζ-potential, morphology, drug-encapsulation efficiency, drug loading, physical stability characterized. The in vitro was tested during planktonic biofilm growth albicans. mean particle 45.62±0.53 nm, they exhibited spheroid-like smooth surfaces, ζ-potential -0.69±0.03 mV. Encapsulation loading 75.37%±2.65% 3.77%±0.13%, respectively. Physical results revealed that despite low measured dispersion stable their 3-week storage at 4°C. minimum inhibitory concentration identical Vrc. However, inhibition rate lower concentrations significantly higher than yeast-extract peptone dextrose medium. Surprisingly, reduced cell density induced more switches form hyphal cells yeast compared with treatment. conclusion, maintain Therefore, will greatly contribute diseases caused