Quantitative analysis for solid-phase hybridization reaction and binding reaction of DNA binder to hybrids using a quartz crystal microbalance

摘要: Abstract A 20-mer DNA probe complementary to a part of an oncogene v-myc, having mercaptohexyl group at the 5′-end, was immobilized on gold electrode quartz crystal microbalance (QCM). The quantity depended pH. Under acidic or basic immobilization conditions, more than under neutral conditions. largest target containing v-myc hybridized when Using QCM which increased with probe. In region where saturated probes, around 70% given hybridized, and ratio target/probe about 1:100. After hybridization, QCMs were reacted Hoechst 33258 (a binder), bound hybrids formed QCMs. target, anodic current derived from linear sweep voltammetry also target. These results suggested that could be applied quantitative analysis for solid-phase hybridization binding binder hybrids.