Regulation of regulators of G protein signaling mRNA expression in rat brain by acute and chronic electroconvulsive seizures.

摘要: G protein-coupled receptor (GPCR) signaling cascades may be key substrates for the antidepressant effects of chronic electroconvulsive seizures (ECS). To better understand changes in these pathways, alterations levels mRNA's encoding regulators G protein (RGS) subtypes-2, -4, -7, -8 and -10 were evaluated rat brain using northern blotting situ hybridization. In prefrontal cortex, RGS2 mRNA increased several-fold 2 h following an acute ECS. Increases RGS8 lesser magnitude (30%), no evident other RGS subtypes. At 24 h a ECS regimen, RGS4, reduced by 20–30%; only RGS10 was significantly after Levels unchanged either or hippocampus, markedly More modest increases seen RGS4 expression, whereas subtypes unaltered. ECS, RGS7, decreased granule cell layer, RGS7 pyramidal layers. Only hippocampus Paralleling neocortex, content ventromedial hypothalamus, The hypothalamus significant These studies demonstrate subtype-, time-, region-specific regulation proteins adaptations that contribute to this treatment.