Biochemical characterization of regulatory factors derived from T cell hybridomas and spleen cells. II. Evidence for glycosylation of T cell growth factor, T cell replacing factor, and granulocyte-macrophage colony-stimulating factor

摘要: Isoelectric focusing demonstrated that T cell growth factor (TCGF), replacing (TRF), and granulocyte-macrophage colony-stimulating (GM-CSF) derived from concanavalin A-stimulated hybridomas spleen cells are heterogeneous with respect to charge. The cell-derived TCGF TRF activities focused isoelectric points (pI) between 3.5 6.5 whereas the range for GM-CSF activity was broader (pI, 8.0). hybridoma-derived were slightly more acidic. Neuraminidase treatment of both hybridoma 123 material resulted in a major peak each (TRF/TCGF pI, 4.9; 4.7). T6-derived peaks around 6.0 as well one 5.0, suggesting this charge heterogeneity due causes other than variations level sialic acid on relevant molecules. Tunicamycin-treated or released biologically active TCGF, TRF, GM-CSF. Each these three tunicamycin-treated pI 5.0. A fraction bound wheat-germ agglutinin. also lentil lectin. These results suggest molecules responsible GM-SCF glycosylated observed lectin-binding characteristics is part variable glycosylation. Glycosylation not critical any biologic activities. No conclusive separation experiments although differed it Concanavalin A.