Production, characterization, and interspecies reactivities of monoclonal antibodies against human class A macrophage scavenger receptors.

摘要: Abstract Class A macrophage scavenger receptor (SR-A) is one of the major receptors macrophages and plays important roles in atherogenesis host defense mechanisms. To assess role SR-A, monoclonal antibodies were generated by immunizing SR-A-deficient mice with a recombinant protein human type I SR-A as immunogen. Four (SRA-C6, SRA-D10, SRA-E5, SRA-F8) confirmed to be specific for Western blot analysis. In early atherosclerotic lesions, these recognized scattered intima foamy periphery atheromatous cores. Interestingly, core lesion only weakly stained. other organs, tissue such alveolar macrophages, Kupffer cells liver, red pulp spleen, sinus lymph nodes, interstitial various organs. Perivascular brain (Mato cells) also positive antibodies. Freshly isolated blood monocytes negative; however, they became after 1 day culture. At 3–5 days culture, reaction intensity stronger along their differentiation towards macrophages. Dendritic interdigitating lymphoid tissues epidermal Langerhans invariably negative. animal tissues, each antibody showed unique pattern. Among four antibodies, SRA-E5 molecules all species examined, including rats mice. These will useful tools study pathological conditions humans species.