Hypoxia upregulates the synthesis of tgf-β1 by human dermal fibroblasts

摘要: Abstract In this report, we have investigated the secretion and synthesis of transforming growth factor-β1 (TGF-β1) by human dermal fibroblast cultures in response to hypoxia (2% oxygen), compared it standard oxygen culture conditions (15% at cell surface). Sandwich enzymelinked immunosorbent assay (SELISA) showed a selective progressive increase TGF-β1 isoform hypoxia, up ninefold after were exposed low for 72 h; TGF-β2 peptide levels not increased. We then transcriptional regulation gene tensions. first 24–48 h, mRNA decreased steadily both environments. This decline continued h but tension. At steady-state 8 times greater oxygen, was reversible upon re-exposure tension 24 h. Elevated m-RNA declined with same half-life addition actinomycin D, suggesting that increased transcription rather than stability. conclude upregulates fibroblasts, leads peptide.