Lysophosphatidylcholine Containing Docosahexaenoic Acid at the sn-1 Position is Anti-inflammatory

摘要: Lysophosphatidylcholine is known to be a lipid mediator in various cellular responses. In this study, we examined the anti-inflammatory actions of lysophosphatidylcholine containing docosahexaenoic acid esterified at sn-1 position. First, RAW 264.7 cells, DHA-lysoPtdCho suppressed LPS-induced formation NO concentration-dependently. However, ARA-lysoPtdCho showed partial suppression, and LNA-lysoPtdCho had no significant effect. Additionally, also reduced level TNF-α or IL-6, but not PGE2. animal experiments, i.v. administration (150 500 μg/kg) prevented zymosan A-induced plasma leakage remarkably with maximal efficacy (Emax) 50%, contrast effect LNA-lysoPtdCho. Remarkably, an ED50 value 46 μg/kg Emax around 95%. mechanistic studies indicated that action was partially related LTC4, TNF-α, IL-6. When interval time between lysoPtdCho A challenge extended up 2 h, such suppressive augmented, suggesting metabolite important for action. support this, 17-HPDHA-lysoPtdCho greater than DHA-lysoPtdCho. Furthermore, similar observed i.p. 17(S)-hydroperoxy derivative. oral expressed Taken together, it proposed its metabolites may lipids vivo systems.