Engineered endothelial cell adhesion via VCAM1 and E-selectin antibody-presenting alginate hydrogels.
作者： Marjan Rafat , Lisa S. Rotenstein , Jennifer L. Hu , Debra T. Auguste
DOI: 10.1016/J.ACTBIO.2012.04.010
关键词:
摘要: Materials that adhere to the endothelial cell (EC) lining of blood vessels may be useful for treating vascular injury. Cell adhesion molecules (CAMs), such as leukocyte molecule-1 (E-selectin) and (VCAM1), modulate EC-leukocyte interactions. In this study, we mimicked cell-cell interactions by seeding cells on alginate hydrogels modified with antibodies against E-selectin VCAM1, which are upregulated during inflammation. ECs were activated interleukin-1α increase CAM expression subsequently seeded onto hydrogels. The strength gels was assessed via a centrifugation assay. Strong, cooperative EC observed presenting 1:1 ratio anti-VCAM1:anti-E-selectin. stronger dual functionalized than anti-VCAM1, anti-E-selectin or arginine-glycine-aspartic acid (RGD) peptide alone. Anti-VCAM1:anti-E-selectin-modified engineered endothelium cooperatively.
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