GeneXpert technology. A breakthrough for the diagnosis of tuberculous pericarditis and pleuritis in less than 2 hours.
作者： Muhammad Saeed , Mobeen Ahmad , Shagufta Iram , Saba Riaz , Mehwish Akhtar
DOI: 10.15537/SMJ.2017.7.17694
关键词:
摘要: Objectives : To evaluate the diagnostic validity of GeneXpert for detection Mycobacterium tuberculosis (MTB) in pericardial and pleural effusions samples. Methods A cross sectional study was conducted at Mycobacteriology Laboratory, Allama Iqbal Medical College, Lahore, Pakistan. total 286 (158 128 fluids) samples were received from (TB) suspects between January 2014 August 2016. Every sample processed Ziehl-Neelsen (Zn) smear, Lowenstein Jensen (LJ) culture, MTB/RIF assay according to standard protocols. Validity MTB evaluated using LJ culture as gold standard. Results Out samples, isolated by 51 (17.8%) followed 43 (15%), acid- fast bacilli (AFB) detected Zn smear microscopy 11 (3.8%) showed high sensitivity (84.3%), specificity (100%), with positive predictive value and negative (96.7%), while 18.3%, 99.1%, 81.8%, value 85.4%. strikingly 72.2% observed fluid GeneXpert. Conclusion is an innovative tool, prompt drug resistance. It definitely attractive point care test, along turn around time 2 hours, which facilitates timely diagnosis appropriate management TB pleuritis pericarditis. Saudi Med J 2017; Vol. 38 (7): 699-705 doi: 10.15537/smj.2017.7.17694 How cite this article: Saeed M, Ahmad Iram S, Riaz Akhtar Aslam M. GeneXpert technology. breakthrough tuberculous pericarditis less than hours. . 2017 Jul;38(7):699-705. 10.15537/smj.2017.7.17694.
smj.org.sa 
core.ac.uk 参考文章(15)
G J Churchyard, V N Chihota, K Fielding, A D Grant, D Muirhead, A van Zyl, B Ndibongo, Liquid vs. solid culture for tuberculosis: performance and cost in a resource-constrained setting International Journal of Tuberculosis and Lung Disease. ,vol. 14, pp. 1024- 1031 ,(2010)
Shumaila N. M. Hanif, Hana S. Eldeen, Suhail Ahmad, Eiman Mokaddas, GeneXpert® MTB/RIF for rapid detection of Mycobacterium tuberculosis in pulmonary and extra-pulmonary samples. International Journal of Tuberculosis and Lung Disease. ,vol. 15, pp. 1274- 1275 ,(2011) , 10.5588/IJTLD.11.0394
Gesine Meyer-Rath, Kathryn Schnippel, Lawrence Long, William MacLeod, Ian Sanne, Wendy Stevens, Sagie Pillay, Yogan Pillay, Sydney Rosen, The Impact and Cost of Scaling up GeneXpert MTB/RIF in South Africa PLoS ONE. ,vol. 7, pp. e36966- ,(2012) , 10.1371/JOURNAL.PONE.0036966
Sanjay Tyagi, Fred Russell Kramer, Molecular Beacons: Probes that Fluoresce upon Hybridization Nature Biotechnology. ,vol. 14, pp. 303- 308 ,(1996) , 10.1038/NBT0396-303
Viral Vadwai, Catharina Boehme, Pamela Nabeta, Anjali Shetty, David Alland, Camilla Rodrigues, Xpert MTB/RIF: a New Pillar in Diagnosis of Extrapulmonary Tuberculosis? Journal of Clinical Microbiology. ,vol. 49, pp. 2540- 2545 ,(2011) , 10.1128/JCM.02319-10
C. M. Denkinger, S. G. Schumacher, C. C. Boehme, N. Dendukuri, M. Pai, K. R. Steingart, Xpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis: a systematic review and meta-analysis European Respiratory Journal. ,vol. 44, pp. 435- 446 ,(2014) , 10.1183/09031936.00007814
M. Coleman, L. J. Finney, D. Komrower, A. Chitani, J. Bates, G. A. Chipungu, E. Corbett, T. J. Allain, Markers to differentiate between Kaposi's sarcoma and tuberculous pleural effusions in HIV-positive patients. International Journal of Tuberculosis and Lung Disease. ,vol. 19, pp. 144- 150 ,(2015) , 10.5588/IJTLD.14.0289
Shaheen Pandie, Jonathan G Peter, Zita S Kerbelker, Richard Meldau, Grant Theron, Ureshnie Govender, Mpiko Ntsekhe, Keertan Dheda, Bongani M Mayosi, Diagnostic accuracy of quantitative PCR (Xpert MTB/RIF) for tuberculous pericarditis compared to adenosine deaminase and unstimulated interferon-γ in a high burden setting: a prospective study BMC Medicine. ,vol. 12, pp. 101- 101 ,(2014) , 10.1186/1741-7015-12-101
Souad M. Al-Ateah, Maha M. Al-Dowaidi, Noura A. El-Khizzi, Evaluation of direct detection of Mycobacterium tuberculosis complex in respiratory and non-respiratory clinical specimens using the Cepheid Gene Xpert® system Saudi Medical Journal. ,vol. 33, pp. 1100- 1105 ,(2012)
Xiaomeng Xu, Weiting Li, Zikun Huang, Qing Luo, Jinghui Du, Junming Li, Xu Liu, Guoliang Xiong, Rapid diagnosis of pleural tuberculosis by Xpert MTB/RIF assay using pleural biopsy and pleural fluid specimens. Journal of Research in Medical Sciences. ,vol. 20, pp. 26- 31 ,(2015)