Characterisation and Separation of Petunia Hybrida and Lycopersicon Esculentum Protoplasts Based on Nutural Fluorescence or FITC Added to Labell Antibodies in Immunoreaction with the Protoplast Membrane

摘要: Although high rates of success have been obtained for intra or inter specific somatic hybridation (1), this has not the case hybridations between genera members different families where it would seem indispensable to a way sorting heterofusions. J.M. WIDHOIM (2) drown up list selection systems fusion products. It seems that available are either diffisult use, like creation resistances, very efficient as example manual sorting. The system by flow cytometry developped K. REDEN-BROUGH (3) two advantages: rare events, and use all categories protoplasts. is based on cell fluorescence. Two applications possible: using natural fluorescence, extinguishing with filters adding another type