Flow cytometry and plant protoplast cell biology
作者： Catherine Bergounioux , Spencer C. Brown , Patrice X. Petit
DOI: 10.1111/J.1399-3054.1992.TB04750.X
关键词: Biochemistry 、 Genetics 、 Absolute calibration 、 Cell function 、 Biology 、 Negative selection 、 Flow cytometry 、 Protoplast
摘要: Flow cytometry, which requires individualized or single cell preparations, is particularly adapted to studies of protoplast biology. It has been developed at three levels: descriptions populations, measurement function, and positive car negative selection rare types. enables several parameters in protoplasts a high rate analysis. Without purification, excellent resolution subpopulations still possible. The quantitative data obtained can each normally have relative units but absolute calibration intensity scale available
researchgate.net 
sci-hub.se 参考文章(29)
David W. Galbraith, Isolation and flow cytometric characterization of plant protoplasts. Methods in Cell Biology. ,vol. 33, pp. 527- 547 ,(1990) , 10.1016/S0091-679X(08)60552-X
C. Bergounioux, C. Perennes, Z. Tabaeizadeh, P. Prevot, P. Gadal, Characterisation and Separation of Petunia Hybrida and Lycopersicon Esculentum Protoplasts Based on Nutural Fluorescence or FITC Added to Labell Antibodies in Immunoreaction with the Protoplast Membrane Experientia. Supplementum. ,vol. 45, pp. 262- 263 ,(1983) , 10.1007/978-3-0348-6556-2_115
David W. Galbraith, Analysis of Higher Plants by Flow Cytometry and Cell Sorting International Review of Cytology-a Survey of Cell Biology. ,vol. 116, pp. 165- 228 ,(1989) , 10.1016/S0074-7696(08)60640-3
Catherine Bergounioux, Spencer C. Brown, Plant cell cycle analysis with isolated nuclei. Methods in Cell Biology. ,vol. 33, pp. 563- 573 ,(1990) , 10.1016/S0091-679X(08)60554-3
K. Arumuganathan, J. P. Slattery, S. D. Tanksley, E. D. Earle, Preparation and flow cytometric analysis of metaphase chromosomes of tomato. Theoretical and Applied Genetics. ,vol. 82, pp. 101- 111 ,(1991) , 10.1007/BF00231283
E. D. Ananiev, L. K. Karagyozov, E. N. Karanov, Effect of cytokinins on ribosomal RNA gene expression in excised cotyledons of Cucurbita pepo L. Planta. ,vol. 170, pp. 370- 378 ,(1987) , 10.1007/BF00395029
Catherine Bergounioux, Claudette Perennes, Spencer C. Brown, Pierre Gadal, Cytometric analysis of growth-regulator-dependent transcription and cell-cycle progression in Petunia protoplast cultures. Planta. ,vol. 175, pp. 500- 505 ,(1988) , 10.1007/BF00393071
David W. Galbraith, Microfluorimetric quantitation of cellulose biosynthesis by plant protoplasts using Calcofluor White Physiologia Plantarum. ,vol. 53, pp. 111- 116 ,(1981) , 10.1111/J.1399-3054.1981.TB04119.X
A. M. M. de Laat, J. Blaas, Flow-cytometric characterization and sorting of plant chromosomes. Theoretical and Applied Genetics. ,vol. 67, pp. 463- 467 ,(1984) , 10.1007/BF00263414
Deborah L. Berglund, Scott Strobel, Fumio Sugawara, Gary A. Strobel, Flow cytometry as a method for assaying the biological activity of phytotoxins Plant Science. ,vol. 56, pp. 183- 188 ,(1988) , 10.1016/0168-9452(88)90033-7