Continuous fermentative hydrogen production from cheese whey wastewater under thermophilic anaerobic conditions

摘要: Abstract Hydrogen (H 2 ) production from cheese processing wastewater via dark anaerobic fermentation was conducted using mixed microbial communities under thermophilic conditions. The effects of varying hydraulic retention time (HRT: 1, and 3.5 days) especially high organic load rates (OLR: 21, 35 47 g chemical oxygen demand (COD)/l/day) on biohydrogen in a continuous stirred tank reactor were investigated. biogas contained 5–82% (45% average) hydrogen the rate ranged 0.3 to 7.9 l H /l/day (2.5 l/l/day average). yields 22, 15 5 mmol/g COD (at constant influent 40 g/l) achieved at HRT values 3.5, 2, 1 days, respectively. On other hand, monitored be 3, 9 6 mmol/g COD, for OLR 47, 21 g COD/l/day, when kept 1 day. total measurable volatile fatty acid concentration effluent (as function COD) between 118 27,012 mg/l, which mainly composed acetic acid, iso-butyric butyric propionic formate lactate. Ethanol acetone also time. To characterize community bioreactor different HRTs, DNA liquor samples extracted immediately PCR amplification 16S RNA gene eubacterial primers corresponding 8F 518R. product cloned subjected sequencing. sequencing results analyzed by MegaBlast available NCBI website showed 99% identity uncultured Thermoanaerobacteriaceae bacterium.