High-Performance Liquid Chromatographic Assay for Sodium Levothyroxine in Tablet Formulations: Content Uniformity Applications

摘要: Sodium levothyroxine was quantitated in 25-300 micrograms/tablet formulations. The procedure consisted of pulverization a suitable sample, extraction into acetonitrile-water (40:60, v/v) containing 0.05% o-phosphoric acid, and injection onto bonded-phase cyanopropyl column; the effluent monitored by UV detection at 225 nm. Spiked placebo recovery studies demonstrated linearity method over range 80-120% label claim. Stability indicated that no degradation products or excipients interfered with quantitation intact drug. Data demonstrating accuracy precision this assay are presented, applied to measurement single-tablet content uniformity.