作者: Alan B. Bennett , Ellen M. Klann , Coralie C. Lashbrook , Serge Yelle , Roger T. Chetelat
DOI: 10.1016/B978-0-7506-9259-5.50013-3
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摘要: Fruit quality is determined by the amount and composition of soluble insoluble solids. In tomato fruit solids are comprised primarily mono disaccharides, whereas cell wall polysaccharides. To assess feasibility improving manipulating monogenic traits, genetic molecular manipulation carbohydrate has been carried out. A trait reversing monosaccharide to disaccharide ratio in transferred from Lycopersicon chmielewskii esculentum shown be governed a single recessive gene. Biochemical characterization this led tentative cloning gene that may control trait, thus providing means genetically engineer one aspect sugar composition. Insoluble polysaccharides, including pectins hemicelluloses, degraded during ripening. manipulate extent timing change polymer structure, we have cloned genes encoding pectin hemicellulose-degrading enzymes using these modify their expression transgenic plants. Together, experiments basis achieving incremental enhancement complex traits components identifying specific biochemical targets for manipulation.