Bean Abscission Cellulase: Characterization of a cDNA Clone and Regulation of Gene Expression by Ethylene and Auxin

摘要: The physiology and anatomy of abscission has been studied in considerable detail; however, information on the regulation gene expression limited because a lack probes for specific genes. We have identified sequenced 595 nucleotide bean (Phaseolus vulgaris cv Red Kidney) cellulase cDNA clone (pBACl). extensive nucleic amino acid sequence identity with avocado pAV363. 2.0 kilobase mRNA complementary to pBACl codes polypeptide approximately 51 kilodalton (shown by hybrid-selection followed vitro translation). Bean antiserum is shown immunoprecipitate from translation products zone poly(A)+ RNA. Ethylene initiates leaf tissue-specific mRNA. If ethylene treatment explants was discontinued after 31 h then 2,5-norbornadiene given inhibit responses resulting endogenously synthesized ethylene, polysomal hybridizing decreased. Thus, required not only initiate but also maintain continued accumulation Explants treated auxin 4 hours prior 48 hour showed no substantial RNA or activity.