Effect of liquid culture on the growth and development of miniature rose (Rosa chinensis Jacq. ‘Minima’)

摘要: The growth of miniature rose (Rosa chinensis Jacq. ‘Minima’) shoots cultured on liquid medium was greater relative to those two-phase (solid + liquid) or solid alone. Shoot multiplication ratio (number multiple per explant subculture) higher with 17.8–26.6 μM 6-benzyladenine at compared that 0–8.9 μM. Shoots grown 30 ml more had a than 10 20 ml. and increased when the culture period extended from 3 6 weeks, although plantlets began exhibit some chlorosis by 6th week. These conditions were maintained over four subcultures for cultivars ‘Baby Katie’, ‘Lavender Jewel’, ‘Red Sunblaze’ ‘Royal Sunblaze’, no significant change in time.