4.2 Interaction of Bacteria and their Products with Tissues in Organ Culture

摘要: Publisher Summary This chapter discusses the interaction of bacteria and their products with tissues in organ culture. Bacterial interactions mucosal surfaces are thought to be critical pathogenesis infectious disease. In order colonize a surface potential pathogen must overcome local defenses, which might include physical barriers, antibacterial substances, resident phagocytes. also facilitate colonization process by compromising defenses. adherence involves specific between structures on bacterial receptors mucosa, although non-specific host cells involving charge hydrophobicity likely. Further, explains advantages cultures. Organ cultures maintain three-dimensional arrangement folds grooves present some epithelial vivo possess population leukocytes, may important during infection, longevity this cell is limited. sufficiently sensitive discriminate closely related strains hence provide powerful tool for investigating effects toxins or importance structures. The number infecting an culture can estimated culturing homogenates tissue agar radioisotope techniques. useful method examining complex under controlled conditions.