Specificity of kidney-bound antibodies in Goodpasture's syndrome.

摘要: The specificity of kidney-bound antibodies in Goodpasture's syndrome was studied and compared with the circulating anti-glomerular basement membrane (GBM) antibodies. Antibodies were eluted from kidneys two patients one normal human kidney. Their by indirect immunofluorescence microscopy, ELISA immunoblotting using purified GBM components as antigens. It shown that reactivity very similar to those anti-GBM Both showed major against M2 subunit globular domain collagen IV. Competitive inhibition binding 125I-labelled serum a patient Goodpasture antigen their respective sera further supported have specificity. These observations extend support clinical usage plasmapheresis more recently, immunoadsorption staphylococcal protein A effective therapeutic measure for removal management syndrome.