An immortalized cell line with features of human follicular dendritic cells. Antigen and cytokine expression analysis

摘要: Follicular dendritic cells (FDC) are specialized residing primarily within lymphoid follicles. They bind immunocomplexes and play an important role in the presentation of antigen to follicular B cells. Isolation FDC for vitro studies, however, is difficult because they constitute only about 1% tissue form tight clusters entrapping lymphocytes their processes. The monoclonal antibody (mAb) Ki-M4, which highly restricted its binding FDC, used identify these cells. In order establish a new immortalized cell line with features we applied modified procedure isolate enrich from human tonsils fused them myeloma SP2/0-Ag14. hybrid line, designated FDC-H1, both mouse origin. FDC-H1 was found have unlimited growth potential consistently express Ki-M4 other surface antigens FDC. Semiquantitative reverse-transcribed polymerase chain reaction (RT-PCR) enriched revealed same cytokine/mRNA profile both, detectable levels interleukin (IL)-1α CD23 lack mRNA IL-1β, IL-2, IL-3, IL-4, IL-7, IL-9, IL-10, interferon-γ, tumor necrosis factor-α, transforming factor-β granulocyte/macrophage-colony-stimulating factor. Additionally weak but constant IL-6 expression by RT-PCR. In situ hybridization experiments transcripts staining pattern characteristic few germinal centers. To our knowledge, first that constantly expresses cytokine FDC. It is, therefore, well suited studying biology functional relationship between normal or neoplastic lymphatic