Is Vasa such a highly specific marker for primordial germ cells? A comparison of VASA and HSP90 proteins expression in young chicken embryos
作者： M. Lejong , M. Choa-Duterre , N. Vanmuylder , S. Louryan
DOI: 10.1016/J.MORPHO.2020.01.003
关键词:
摘要: Summary Introduction Primordial germ cells (PGCs) have been studied since the 19th century with several different methods. The earliest works were based on morphological criteria of these associated or not a particular staining. Different markers proposed in immunohistochemistry among which we can quote Stage-specific embryonic antigene-1 (SSEA-1), mouse antigen-1 (EMA-1) heat shock protein 90. Unfortunately, none them are germline specific. VASA is considered as one most reliable marker for PGCs by some authors its expression being to limited cells. However, other studies reported somatic Here, described protein, HSP90, and early chick embryo. Material methods Embryos from stages Hamburger-Hamilton (HH) 19, 21 28 collected. dissected fixed Serra's medium. Sections placed slides PAS staining double HSP90 VASA. Results observed but well cell lineages spatio-temporal gradient respect characteristics development each organ. conclusion that line
sci-hub.se 参考文章(30)
Suzanne L. Rutherford, Susan Lindquist, Hsp90 as a capacitor for morphological evolution Nature. ,vol. 396, pp. 336- 342 ,(1998) , 10.1038/24550
M. Yajima, G. M. Wessel, Essential elements for translation: the germline factor Vasa functions broadly in somatic cells Development. ,vol. 142, pp. 1960- 1970 ,(2015) , 10.1242/DEV.118448
D. H. Castrillon, B. J. Quade, T. Y. Wang, C. Quigley, C. P. Crum, The human VASA gene is specifically expressed in the germ cell lineage. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 97, pp. 9585- 9590 ,(2000) , 10.1073/PNAS.160274797
Nathalie Vanmuylder, Aimée Werry-Huet, Marcel Rooze, Stéphane Louryan, Heat shock protein HSP86 expression during mouse embryo development, especially in the germ-line. Anatomy and Embryology. ,vol. 205, pp. 301- 306 ,(2002) , 10.1007/S00429-002-0258-5
Daniel E. Wagner, Jaclyn J. Ho, Peter W. Reddien, Genetic regulators of a pluripotent adult stem cell system in planarians identified by RNAi and clonal analysis. Cell Stem Cell. ,vol. 10, pp. 299- 311 ,(2012) , 10.1016/J.STEM.2012.01.016
Toshiaki Noce, Shino Okamoto-Ito, Naoki Tsunekawa, Vasa Homolog Genes in Mammalian Germ Cell Development Cell Structure and Function. ,vol. 26, pp. 131- 136 ,(2001) , 10.1247/CSF.26.131
Paul Lasko, The DEAD-box helicase Vasa: Evidence for a multiplicity of functions in RNA processes and developmental biology Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. ,vol. 1829, pp. 810- 816 ,(2013) , 10.1016/J.BBAGRM.2013.04.005
Lynne Hamburger Shevinsky, Barbara B. Knowles, Ivan Damjanov, Davor Solter, Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen expressed on mouse embryos and human teratocarcinoma cells Cell. ,vol. 30, pp. 697- 705 ,(1982) , 10.1016/0092-8674(82)90274-4
Lisbeth Charlotte Olsen, Rein Aasland, Anders Fjose, A vasa-like gene in zebrafish identifies putative primordial germ cells. Mechanisms of Development. ,vol. 66, pp. 95- 105 ,(1997) , 10.1016/S0925-4773(97)00099-3
Niles Fox, Ivan Damjanov, Antonio Martinez-Hernandez, Barbara B. Knowles, Davor Solter, Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues Developmental Biology. ,vol. 83, pp. 391- 398 ,(1981) , 10.1016/0012-1606(81)90487-5