Rapid mapping of a chlorina mutant gene cn-A1 in hexaploid wheat by bulked segregant analysis and single nucleotide polymorphism genotyping arrays

摘要: The yellow–green leaf mutant can be exploited in photosynthesis and plant development research. A Triticum aestivum with the chlorina phenotype, here called B23, was produced by treatment chemical mutagen sodium azide. This B23 showed significantly lower chlorophyll content than wild-type Saannong33, its chloroplast structure abnormal. All yield-related traits, except for number of spikes per plant, were also decreased. Genetic analysis confirmed that phenotype controlled a recessive gene, designated cn-A1. Using bulked segregant wheat 660K single nucleotide polymorphism array, cn-A1 gene mapped to chromosome 7AL, 11 polymorphic markers developed. Further located 1.1-cM genetic region flanked Kompetitive allele specific PCR (KASP) 660K-7A12 660K-7A20, which corresponded physical interval 3.48 Mb T. cv. Chinese Spring 7AL containing 47 predicted genes high confidence. These results are expected accelerate process cloning facilitate understanding mechanisms underlying metabolism wheat.