Quantification of human Alu sequences by real-time PCR – an improved method to measure therapeutic efficacy of anti-metastatic drugs in human xenotransplants

摘要: For measuring the efficacy of new anti-metastatic drugs in preclinical models, macroscopical analysis or classical histology secondary organs are established methods. However, evaluation does not take into consideration intra-organ metastasis. Histological is often performed few sections relevant organs, and this may be misleading, since equal distribution tumor cells within an organ unlikely. In addition, recent studies have demonstrated that anti-tumorigenic able to promote metastasis change metastatic pattern. Therefore, extensive mandatory for compounds. A feasibility study was conducted find out if quantification human Alu sequences could applied as a surrogate marker xenografts. PCR by using LightCycler® system,* which allows reaction subsequent products less than 30 min. We found i) equivalent one cell 1 × 106 murine detected; ii) tumor-carrying mice, signal increased over time organs; iii) increase more prominent highly cells; iv) intensity DNA extracted from tissue slides correlated with expression histological markers; v) three different models (colon, breast lung), treatment Taxol 5-fluorouracil reduced amount organs. contrast, reduction matrix metalloproteinase inhibitor RO 28-2653 significant. Taken together, fast accurate method evaluate therapeutic