Genetic Evaluation of TLR4 and CaLA-DRB Genes for Mastitis Susceptibility in Dairy Goats and the Response of Mastitis-Bacteria to Garlic and Black pepper Extracts

摘要: MOLEFE PETLANE. D151108141. Genetic Evaluation of TLR4 and CaLADRB Genes for Mastitis Susceptibility in Dairy Goats the Response Mastitis-Bacteria to Garlic Black pepper Extracts. Supervised by RONNY R. NOOR RARAH R.A. MAHESWARI. is a bacterial disease that affects mammary glands nursing animals. It results serious production economic losses due costs attached its treatment loss animals through culling. This study was aimed at evaluating CaLA-DRB genes mastitis susceptibility Saanen, PE PESA using PCR-RFLP also investigate antibacterial effects garlic black extracts on causing bacteria. transmembrane protein recognizes PAMPs, convey signals into nucleus thus transcription factors inhibit primary infection while DRB cell surface antigens present such T-cells CD4. Allicin an active ingredient garlic; it confers antibiotic properties interfering with polymerase enzyme system inhibiting formation nucleic acids whereas piperine alkaloid forms pepper. Piperine alters permeability membrane inhibits NorA efflux pump mechanism leakage accumulation extracellular substances which may cause death. A total 68 lactating dairy goats; consisting from Balitnak, Caprito farm, Cordero farm Bangun were used this study, population consisted both mastitic healthy polymorphism detected digesting PCR product AluI amplicon digested PstI TaqI two separate reactions. Antimicrobial tests performed agar well diffusion technique. The found TLR4|AluI locus monomorphic fixed allele “T” all breeds analyzed. Allele P had high frequency (0.7069) followed (0.6900) then Saanen (0.6705) T (0.9205), (0.8976) (0.7188). DRB|PstI DRB|TaqI loci polymorphic where exhibited most heterozygosity. moderate PIC low PESA. χ 2 indicated exception still Hardy-Weinberg equilibrium. Staphylococcus aureus frequently (80%) isolated bacteria Escherichia coli less frequent (<30%), leading decision S.aureus main etiological agent caprine mastitis. genotypes are susceptible but genotype PP TT comparatively more resistant as compared other since they displayed higher against genotypes. Antibacterial revealed E.coli sensitive