Detection of neuronal tissue in meat using tissue specific DNA modifications

摘要: A method has been developed to differentiate between non-muscle tissues such as liver, kidney and heart that of muscle in meat samples using tissue specific DNA detection. Only is considered from the point view labelling (Food Labelling [Amendment] (England) Regulations 2003) Quantitative Ingredient Declaration (QUID), also certain parts carcass are prohibited be used raw products (Meat Products [England] 2003). Included offal brain spinal cord. The described methodology therefore primarily enforce rules but contribute enforcement BSE legislation on detection Central Nervous System (CNS) tissue. latter requires removal Specified Risk Material (SRM), bovine ovine cord, food chain. Current methodologies for CNS include histological examination, analysis cholesterol content immunodetection. These can potentially time consuming, less applicable processed may not readily adapted high throughput sample analysis. objective this work was develop a DNAbased assay exploits sensitivity specificity PCR more highly samples. For neuronal tissue, target selected promoter Glial Fibrillary Acidic Protein (GFAP), gene whose expression restricted astroglial cells within fragments both cattle sheep have isolated key differences methylation patterns CpG dinucleotides sequences cord corresponding skeletal identified. design exploiting Methylation Specific (MSP) specifically amplify derived sequence identify presence an sample.