DNA probes for the identification of Haemophilus ducreyi.

摘要: Haemophilus ducreyi ATCC 33922, a virulent, well-characterized strain, was used to construct genomic library in bacteriophage expression vector. Three DNA fragments were selected for use as probes on the basis of their ability encode H. ducreyi-specific proteins, demonstrated by reactivity with rabbit polyclonal antiserum. With DNA-DNA hybridization, three probes, labeled 32P, reacted strongly 16 strains obtained from variety sources. Thirty-seven other bacterial isolates, representing 33 different species and including organisms likely be encountered urogenital tract, also tested probes. Twenty-eight these genital pathogen Neisseria gonorrhoeae, showed no hybridization In addition, herpes simplex virus-infected tissue culture cells Treponema pallidum-infected testicular fluid completely nonreactive. Nine six belonging Pasteurella species, weakly when approximately 3.0 x 10(7) 6.0 CFU tested. When 10(5) 10(6) tested, weak reactions could longer seen. Yet this number still strongly. fact, consistently detected 10(4) pure mixed cultures even produced signal only 10(3) present. It is clear our results that will greatly facilitate laboratory diagnosis pathogen. Images