Infectivity of Dengue Virus Serotypes 1 and 2 Is Correlated with E-Protein Intrinsic Dynamics but Not to Envelope Conformations

摘要: Summary Dengue is a mosquito-borne virus with dire health and economic impacts. responsible for an estimated 390 million infections per year, dengue 2 (DENV2) being the most virulent strain among four serotypes. Interestingly, it also in strains of this serotype that temperature-dependent large-scale morphological changes, termed “breathing,” have been observed. Although structure of these morphologies has solved to 3.5-A resolution, dynamics viral envelope are unknown. Here, we combine fluorescence mass spectrometry molecular simulations provide insights into DENV2 (NGC strain) structural comparison DENV1 (PVP 159). We observe hitherto unseen conformational changes influenced by both temperature divalent cations. Our results show intrinsic dynamics, but not specific morphologies, correlated infectivity.